Optimasi Rasio Ekstrak Bahan Organik Alami dalam Formulasi Media Kultur untuk Regenerasi In Vitro Aglonema Varietas Red Borju

Citra Permata; Ikhsan Kartika

doi:10.70716/inbio.v1i3.345

Authors

Citra Permata Universitas Gadjah Mada
Ikhsan Kartika

DOI:

https://doi.org/10.70716/inbio.v1i3.345

Keywords:

Aglonema Red Borju , in vitro culture , natural organic media , Allium cepa , Phaseolus radiatus

Abstract

This study aimed to optimize the ratio of natural organic extracts derived from shallot (Allium cepa) and mung bean sprouts (Phaseolus radiatus) in culture media formulations to enhance in vitro regeneration of Aglonema cv. Red Borju. These organic materials are known to contain natural phytohormones, such as auxins and cytokinins, as well as other bioactive compounds that potentially stimulate morphogenesis. The experiment was conducted using several extract ratio treatments incorporated into Murashige and Skoog (MS) basal medium. Growth responses were evaluated based on plantlet height, number of leaves, number of roots, fresh weight, and dry weight. The results demonstrated that variations in extract ratios significantly affected plantlet growth performance. The combination of shallot and mung bean sprout extracts at a ratio of 2:1 produced the most significant improvement in all observed growth parameters. The use of natural organic extracts proved to be an effective alternative to partially replace synthetic components in culture media without reducing growth efficiency. This study contributes to the development of environmentally friendly and cost-effective in vitro culture techniques for the mass propagation of Aglonema cv. Red Borju.

References

Bhojwani, S. S., & Razdan, M. K. (1996). Plant tissue culture: Theory and practice. Elsevier.

Casanova, E., Moysset, L., & Trillas, M. I. (2005). Effects of plant growth regulators and natural additives on in vitro plant development. Plant Cell, Tissue and Organ Culture, 80(1), 1–8. https://doi.org/10.1007/s11240-004-9193-6

George, E. F., Hall, M. A., & De Klerk, G. J. (2008). Plant propagation by tissue culture (3rd ed.). Springer.

Hartmann, H. T., Kester, D. E., Davies, F. T., & Geneve, R. L. (2011). Plant propagation: Principles and practices. Prentice Hall.

Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum, 15, 473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x

Nasir, M., & Faisal, M. (2018). Organic additives in plant tissue culture: A review. International Journal of Agriculture and Biology, 20(5), 1043–1050. https://doi.org/10.17957/IJAB/15.0591

Pierik, R. L. M. (1997). In vitro culture of higher plants. Springer.

Smith, R. H. (2013). Plant tissue culture: Techniques and experiments (4th ed.). Academic Press.

Teixeira da Silva, J. A. (2013). The role of organic additives in plant tissue culture. Plant Growth Regulation, 69(2), 107–121. https://doi.org/10.1007/s10725-012-9765-3

Thorpe, T. A. (2007). History of plant tissue culture. Molecular Biotechnology, 37(2), 169–180. https://doi.org/10.1007/s12033-007-0031-3

Kumar, A., Sharma, S., & Singh, D. (2019). Use of natural extracts in plant tissue culture systems. Journal of Plant Biotechnology, 46(2), 112–120. https://doi.org/10.5010/JPB.2019.46.2.112

Méndez, J., & Roca, W. (1989). Influence of natural substances on plant tissue culture. Plant Cell Reports, 8(1), 22–25. https://doi.org/10.1007/BF00270089